The regulation of denitrification is a complicated process, which is not yet fully understood. In order to gain more insight the regulation of denitrification, we are working on the following topics:
We are investigating the nitric oxide signaling mechanism by trapping NO with iron-containing porphyrin-hemin. The experimental data from anaerobic growth in bottles and microtiter plates suggests that, dependent on the number of viable cells and hemin concentration, the cells would either slow down growth or express a dark pink compound in the medium. We aim at isolation and analysis of this compound.
Identification of transcriptional start sites (TSS) of the denitrification genes and quantification ot their mRNAs is an important aspect in understanding the regulation of the process. In collaboration with UEA, we performed primer extension experiments and discovered that the TSS for the nosR gene is located at the 3’ end of the nosC gene. This result will be verified. Moreover, we aim to identify the potential TSS’s for remaining genes in the nos cluster.
Recent studies suggest that transcriptomes in many prokaryotes are very complex and that genes in an operon are dynamically transcribed at different conditions. We have designed experiments with P. denitrificans grown at four different conditions aiming at getting more insight into its transcriptomes during the adaptive responses.